Development of a Dot-blot System to Detect Gluten in Food
نویسندگان
چکیده
JOSÉ RAMÓN GIL, YOUNES SBIHI, PEDRO ANTONIO ALVAREZ, MIMOUN MAACHE, MANOLO LARRUBIA, JOSÉ ROJAS AND ANTONIO OSUNA Instituto Biotecnologı́a, Departamento de Parasitologı́a, Facultad de Ciencias, Severo Ochoa s/n, Universidad de Granada, 18071, Granada, Spain; Departamento de I /D /I, Sanavi, SA Lachar, Granada, Spain; Departamento de I /D, Vircell, SA Santa Fe, Granada, Spain; Département de Sciences du Vivant, Uria-Cnesten-BP1382-Rabat, Morocco
منابع مشابه
Development of antibody-based microarray assay for quantitative detection of aflatoxin B1
BACKGROUND: Aflatoxin B1 (AFB1) is a toxic metaboliteproduced by Aspergillus species that contaminates a wide range ofagricultural products. OBJECTIVES: This study was designed todevelop a rapid and highly sensitive immunoassay method inmicroarray format for quantitative detection of AFB1 to evaluatethe potential of microarray platform for high-throughput screening,which can be beneficial in fo...
متن کاملDevelopment of a recombinant protein-based dot-blot hybridization assay for the detection of antibody to chicken infectious bronchitis virus
Nucleocapsid (N) protein of infectious bronchitis virus (IBV), one of the viral structural proteins, inducesstrong antibody response in natural infection. In this study, a simple, recombinant N protein-based dot-blottest was developed to serologically examine chicken serum samples for the presence of IBV antibody.Initially, 72 serum samples were tested for the presence of IBV antibody using a c...
متن کاملDesign and optimization of Dot-blot ELISA system using specific antigens for simple and rapid diagnosis of glanders
Introduction Glanders is one of the oldest contagious and dangerous zoonotic diseases manifesting ulcerative granulomatous lesions on the skin and mucous membranes. Early methods possessing desirable sensitivity and specificity is important to diagnose the disease considering the just only one case report and preventing disease by identification and eradication. The present study was aimed to ...
متن کاملModification of physicochemical and nutritional properties of gluten free cakes by natural additives application
The demand for gluten-free products has increased in recent years, owing to the market trends and an increase in the number of celiac and other gluten sensitivities and wheat allergies. Products that contain gluten are also a major diet for a large portion of the world's population. For this reason, the development of gluten-free products is not only an urgent need for celiac patients but also ...
متن کاملAPPLICATION AND ADAPTATION OF DOT IMMUNOASSAY TO DETECT AND MEASURE RUBELLA VIRUS ANTIBODY
A dot immunobinding assay ( DIA) was used for a quantitative and qualitative assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin (RAHlg) were prepared. Nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. The reacting antibodies were visualized by a peroxidase system. Development of a colored insoluble substrate ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2004